増液承气汤对热结肠道型气分证大鼠肠道组织结构、血清IL-1β、IL-6及TNF-α含量的影响

doi:10.11843/j.issn.0366-6964.2016.04.024

摘要/Abstract

摘要：

为了探讨增液承气汤对热结肠道型气分证大鼠肠道组织结构及血清炎性细胞因子IL-1β、IL-6及TNF-α含量的影响，给热结肠道型气分证模型大鼠预防性或治疗性灌服增液承气汤，测定大鼠体温、体重、肠道黏膜组织结构变化和血清相关炎性细胞因子含量。结果表明：热结肠道型气分证大鼠肠道屏障功能受损，肠道黏膜脱落，部分绒毛断裂，炎性细胞浸润，上皮内淋巴细胞（IEL）数量增多，杯状细胞（GC）数量减少，且血清中炎性细胞因子IL-1β、IL-6和TNF-α均会出现显著升高（P<0.05）。预防性给予增液承气汤后，可有效减轻肠道黏膜脱落，绒毛断裂和炎性细胞浸润等损伤，并能有效降低大鼠血清中IL-6和TNF-α含量（P<0.05）；治疗性给予增液承气汤能有效地降低大鼠体温，使大鼠肠道组织结构基本恢复正常，恢复肠道黏膜免疫屏障功能，可有效降低大鼠血清中IL-1β、IL-6和TNF-α含量。增液承气汤可通过降低体温、维护肠道组织结构正常，降低IEL及升高GC细胞数量，抑制血清炎性细胞因子的分泌而起到良好的防治作用。

Abstract:

In order to study the effects of fluid-increasing decoction for purgation on intestinal structure，concentration of IL-1β，IL-6 and TNF-α in serum of rat in heat accumulation of intestinal type of Qi-fen syndrome，the experiments were carried out to construct the heat accumulation of intestinal type of Qi-fen syndrome，and the fluid-increasing decoction for purgation was given to the rat in preventability or therapeutically，then body temperature，the intestinal organizational structure，and the concentration of inflammatory cytokines in serum of rat were also be studied.The results showed that the intestinal structure in rat in heat accumulation of intestinal type of Qi-fen syndrome were significantly changed，for example，the intestinal mucosa was dropped，inflammatory cells were infiltrated，IELs were increased，goblet cells (GC) were decreased，and the concentration of IL-1β，IL-6 and TNF-α in serum were increased in significantly.The rat were given with the fluid-increasing decoction for purgation in preventability，which will alleviate the damages to intestine of rats，and the concentration of IL-1β，IL-6 and TNF-α decreased significantly；and the rat were given the decoction in therapeutically，which will decrease the body temperature，keep the intestinal structure and mucosal barrier in normal，and decrease the concentration of IL-1β，IL-6 and TNF-α in serum of rats.Therefore，fluid-increasing decoction for purgation which is the classic formula to treat the rats in heat accumulation of intestinal type of Qi-fen syndrome has the preventability and therapeutically effects through decreasing the body temperature，maintaining the intestinal structure in normal，decreasing the IEL’ number and increasing the GC’ number，and then inhibition the secretion of inflammation cytokines.

中图分类号:

S853.9

王自力，王晓谦，张翥，涂君平，蒋会会，别应堂，张家骅. 増液承气汤对热结肠道型气分证大鼠肠道组织结构、血清IL-1β、IL-6及TNF-α含量的影响[J]. 畜牧兽医学报, 2016, 47(4): 828-835.

WANG Zi-li，WANG Xiao-qian，ZHANG Zhu，TU Jun-ping，JIANG Hui-hui，BIE Ying-tang，ZHANG Jia-hua. Effects of Fluid-increasing Decoction for Purgation on Intestinal Structure，Concentration of IL-1β，IL-6 and TNF-α in serum of Rat in Heat Accumulation of Intestinal Type of Qi-fen Syndrome[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 828-835.

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参考文献

［1］刘钟杰，许剑琴.中兽医学［M］.3版.北京：中国农业出版社，2002：112-113.
LIU Z J，XU J Q.Traditional Chinese veterinary medicine［M］.3ed.Beijing：China Agriculture Press，2002：112-113.(in Chinese)
［2］尹艳芬，周晋华.增液承气汤临床研究进展［J］.中国中医药现代远程教育，2014，12(16)：163-164.
YIN Y F，ZHOU J H.Research progress of clinical application of Zengye Chengqi decoction［J］.Chinese Medicine Modern Distance Education of China，2014，12(16)：163-164.(in Chinese)
［3］任志兵，温娟，李庆亮，等.增液承气汤治疗5-HT₃受体拮抗剂所致便秘的临床研究［J］.中医临床研究，2014，6(27)：23-25.
REN Z B，WEN J，LI Q L，et al.Clinical study of Zengye Chengqi decoction for the treatment of constipation caused by 5-HT₃ receptor antagonist［J］.Clinical Journal of Chinese Medicine，2014，6(27)：23-25.(in Chinese)
［4］王自力，贺庭祯，张翥，等.大鼠热结肠道型气分证模型的构建及肠道结构、IEL和GC数量的变化研究［J］.畜牧兽医学报，2014，45(4)：672-678.
WANG Z L，HE T Z，ZHANG Z，et al.Study on construction of heat accumulation of intestinal type of Qi-fen syndrome and changes of intestinal structure and number of IEL，GC in rat［J］.Acta Veterinaria et Zootechnica Sinica， 2014，45(4)：672-678.(in Chinese)
［5］张西波，崔乃强，袁红霞，等.阳明腑实证患者内毒素及炎症介质的变化与肠源性内毒素血症的相关性研究［J］.天津中医药，2007，24(3)：187-189.
ZHANG X B，CUI N Q，YUAN H X，et al.Correlation between changes of endotoxin，inflammatory mediators and intestinal endotoxemia in patients with excess syndrome of Yang Ming Fu-organ［J］.Tianjin Journal of Traditional Chinese Medicine，2007，24(3)：187-189.(in Chinese)
［6］周璇，程方平.清热化湿方对温病湿热证模型大鼠血清TNF-α、IL-1β、SIgA的影响［J］.辽宁中医药大学学报，2007，9(2)：142-143.
ZHOU X，CHENG F P.Effects of Qingre Huashi capsule on TNF-α、IL-1β、SIgA concentration in serum dampness-heat model rats［J］.Journal of Liaoning University of Traditional Chinese Medicine，2007，9(2)：142-143.(in Chinese)
［7］MORICHI S，YAMANAKA G，ISHIDA Y，et al.Brain-derived neurotrophic factor and interleukin-6 levels in the serum and cerebrospinal fluid of children with viral infection-induced encephalopathy［J］.Neurochem Res，2014，39(11)：2143-2149.
［8］GÓMEZ C D，BUIJS R M，SITGES M.The anti-seizure drugs vinpocetine and carbamazepine，but not valproic acid，reduce inflammatory IL-1β and TNF-α expression in rat hippocampus［J］. J Neurochem，2014，130(6)：770-779.
［9］HARDEN L M，DU PLESSIS I，POOLE S，et al.Interleukin (IL)-6 and IL-1 beta act synergistically within the brain to induce sickness behavior and fever in rats［J］.Brain Behav Immun，2008，22(6)：838-849.
［10］石玉祥，闫金坤，王雪敏.枸杞多糖对小鼠肠道上皮内淋巴细胞和杯状细胞数量、分布及对IL-2水平影响［J］.食品科学，2011，32(13)：318-320.
SHI Y X，YAN J K，WANG X M.Effect of Chinese wolfberry (Lycium barbarum) polysaccharides on number and distribution of intraepithelial lymphocytes and goblet cells and IL-2 expression in mice［J］. Food Science，2011，32(13)：318-320.(in Chinese)
［11］于征淼，伍军军，赵光峰，等.脂多糖注射法制作兔温病实验模型研究［J］.中华中医药学刊，2010，28(2)：286-288.
YU Z M，WU J J，ZHAO G F，et al.Endue rabbit experimental model of epidemic febrile disease by lipopolysaccharide infusion［J］.Chinese Archives of Traditional Chinese Medicine，2010，28(2)：286-288.(in Chinese)
［12］龚成珍.白虎汤治疗内毒素致家兔温病气分证的实验研究［D］.兰州：甘肃农业大学，2011.
GONG C Z.Experimental study on Baihutang treatment for Qifen syndrome in seasonal febrile disease of endotoxin with rabbits［D］.Lanzhou：Gansu Agricultural University，2011.(in Chinese)
［13］XU R J，WANG F，ZHANG S H.Postnatal adaptation of the gastrointestinal tract in neonatal pigs：a possible role of milk-borne growth factors［J］. Livest Prod Sci，2000，66(2)：95-107.
［14］刘莲莲.仔兔大肠杆菌病小肠黏膜免疫变化及治疗效果观察［D］.泰安：山东农业大学，2012.
LIU L L.Changes of small intestinal mucosal immune of rabbit colibacillosis and comparison of treatment efficiency［D］.Tai’an：Shandong Agricultural University，2012.(in Chinese)
［15］巫全胜，钱宁，吴曙光.药物依赖性便秘大鼠大肠杯状细胞及5-HT变化的实验研究［J］.实验动物科学，2008，25(3)：11-13.
WU Q S，QIAN N，WU S G.An experimental study of the morphological changes of goblet cells and 5-HT immunoreactive cells of intestine of drug-dependent constipation in rats［J］.Laboratory Animal Science，2008，25(3)：11-13.(in Chinese)
［16］徐江红.增液承气汤对家兔排便作用的实验研究［D］.石家庄：河北医科大学，2011.
XU J H.The experimental study of Zengye Chengqi decoction on defecation function in rabbits［D］.Shijiazhuang：Hebei Medical University，2011.(in Chinese)
［17］王文珊，傅冷西，叶君健.TNF-α信号传导通路的研究进展［J］.福建医科大学学报，2005，39(Suppl)：27-31.
WANG W S，FU L X，YE J J.Recent advance of TNF-α signal transduction pathway research［J］.Journal of Fujian Medical University，2005，39(Suppl)：27-31.(in Chinese)
［18］温如果.白虎汤对内毒素性发热家兔解热机制的研究［D］.沈阳：辽宁中医药大学，2009.
WEN R G.The mechanism of Bai-Hu-Tang (BHT) decoction on fever induced by endotoxin(ET)［D］.Shenyang：Liaoning University of Chinese Medicine，2009.(in Chinese)
［19］吴永丰，谢久彬，郭妮.黄连、牡丹皮配伍大承气汤对肠热腑实证大鼠IL-6和NO含量的影响［J］.河南中医，2011，31(9)：988-989.
WU Y F，XIE J B，GUO N.Effects of berberine and cortex moutan compatible with Dachengqi decoction on IL-6 and NO of rats with intestine-heat and Fu-organ excess syndrome［J］.Henan Traditional Chinese Medicine，2011，31(9)：988-989.(in Chinese)
［20］司惠丽，刘春蕾，王世军，等.大黄水煎液对实热证模型大鼠血浆中IL-1β、IL-6、TNF-α水平的影响［J］.山东中医药大学学报，2012，36(1)：58-59.
SI H L，LIU C L，WANG S J，et al.Effects of rheum officinale water extract on IL-1β、IL-6、TNF-α levels in serum of rat in Damp heat syndrome model［J］.Journal of Shandong University of TCM，2012，36(1)：58-59.(in Chinese)

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